AG Scientific Benzamidine Agarose 6B, 25 ML

Description:
Benzamidine Agarose 6B is p-aminobenzamidine covalently attached to Agarose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), isan inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine-Agarose6B and can thus be used for purification and/or removal of these substances.

Benzamidine-Agarose6B can be used for purifying serine proteases such as trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain. For recombinant purification, Benzamidine-Agarose6B can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags.

Benzamiding-Agarose 6B is tested for its binding capacity for trypsin, but the resin has been used in the purification of a variety of proteins including thrombin, Adenylyl cyclase-activating protease from bovine sperm, Fibrinolytic enzyme from Agkistrodon contortrix, Tryptase from rat skin,

Serine endopeptidase from rat mammary tissue, Glycosylation-enhancing factor (GEF), Plasminogen activator from embryo lung culture, Plasminogen activator from human umbilical vein endothelial cells and Thrombin-like enzyme from Bothrops atrox venom

Specifications:

• Ligand: p-Aminobenzamidine (PAB)
• Matrix: Separopore® 6B (agarose beads, 6%)
• Particle size range: 52 – 165 µm
• Matrix activation: Epoxy activation
• Ligand density:7 μmole p-aminobenzamidine/ml drained gel
• Binding capacity: > 10mg trypsin/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• pH stability: Long term:3 – 11; Short term:2 – 13
• Chemical stability: Stable to all commonly used aqueous buffers
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Storage temp: 4 –8 °C
• Benzamidine-Agarose 6B is supplied as pre-swollen gel in 20% ethanol