AG Scientific Streptavidin Agarose 4B, 5 ML

AG Scientific ​​​Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linkedbeaded agarose with high chemical stability. The immobilized streptavidin bindsbiotin and biotinylated substances and can be used for affinity chromatographyapplications. The interaction between streptavidin and biotin is very strong andrequires denaturating conditions for elution, which may destroy both the ligandand the sample.

Streptavidin 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin. It can be used in the purification of antigens, where biotinylatedantibodies are incubated with antigen. The biotinylated antibody-antigencomplex binds to Streptavidin Agarose from which the antigen can be eluted.

Supplied as suspension in buffered saline solution.​​​​

Specifications:

• Ligand: Native Streptavidin (fromStreptomyces avidinii)
• Matrix: agarose beads, 4%
• Particle size range: 52 -165 µm
• Matrix activation: Cyanogen bromide
• Ligand density: 2 mg streptavidin / ml drained gel
• Binding capacity: >20 µg biotin / ml drained gel
• Exclusion limit: ~ 2 x 10⁷
• pH stability: 3 – 10
• Binding buffer: 50 mM ammonium carbonate, 0.5M NaCl, pH 10
• Elution buffer: 50 mM ammonium acetate, 0.5M NaCl, pH 4

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