Sigma-Aldrich Schaeffer and Fulton Spore Stain Kit

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the endospores by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safranin. Endospores are green colored while bacteria show up as red bodies.

Schaeffer & Fulton′s Spore Stain-Kit is used for staining bacterial spores

Kit contents:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water,
Schaeffer & Fulton`s Spore Stain B: Safarin O 5 g/l in water