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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC28 |
| Description | Anti-RIP3 Antibody, clone 6E6.2 |
| Alternate Names |
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| Background Information | Receptor-interacting protein 3 (RIP3) contains a N-terminal kinase domain and interacts with RIP. The binding of RIP to RIP3 causes the RIP3/RIP complex to be shuttled into the tumor necrosis factor receptor-1 signaling cascade. RIP3 is known to be a very effective apoptosis inducer. Two novel splice variants of RIP3 have been discovered, RIP3 beta and RIP3 gamma, that have shown to decrease apoptosis related to full length RIP3. RIP3 gamma has been shown to be significantly up-regulated in colon and lung cancers and may play a role in tumorigenesis. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-RIP3 Antibody, clone 6E6.2 is validated for use in WB, IH for the detection of RIP3. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A 1:1,000 dilution from a representative lot detected RIP3 in 10 µg of human placenta tissue lysate. Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RIP3 in rat pancreas tissue. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human RIP3 at the N-terminus. |
| Epitope | N-terminus |
| Clone | 6E6.2 |
| Host | Mouse |
| Specificity | This antibody recognizes RIP3 at the N-terminus. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Promotes apoptosis. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBUNIT STRUCTURE: Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. SUBCELLULAR LOCATION: Cytoplasm Probable. TISSUE SPECIFICTY: Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. PTM: Autophosphorylated (By similarity). SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
| Molecular Weight | ~56 kDa (isoform 1) and ~27 kDa (isoform 2) observed. Isoform 3 (~25 kDa) may be observed in some lysates. An uncharacterized band may be observed at ~16 kDa in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human pancreas tissue lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected RIP3 in 10 µg of human pancreas tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |