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Cell Signaling Lc3b (D11) Xp Rabbit mAb
Cell Signaling Lc3b (D11) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$152.00
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$152.00
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NETA PART: | CSIG-3868T |
MFG.PART: | 3868T |
UNSPSC: | 41116133 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 14, 16 |
Source/Isotype | Rabbit IgG |
Application/Dilution | {Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200, Flow Cytometry (Fixed/Permeabilized): 1:200} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | LC3B (D11) XP® Rabbit mAb detects endogenous levels of total LC3B protein. Cross-reactivity may occur with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B. |
Species Reactivity | Human |
Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LC3B. |
Background | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). |
SKU | CSIG-3868T |
---|---|
Supplier Part Number | 3868T |
UM | UNIT |
UNSPSC | 41116133 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |