Cell Signaling Survivin (71g4b7) Rabbit mAb

Name: Cell Signaling Survivin (71g4b7) Rabbit mAb
SKU: CSIG-2808T
Price: 146.45 USD

List Price $146.45 Your Price $146.45

Cell Signaling Survivin (71g4b7) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2808T

MFG.PART: 2808T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	16
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:50 - 1:250, Immunoprecipitation: 1:400, Immunohistochemistry (Paraffin): 1:200 - 1:800, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800, Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #56622.
Specificity/Sensitivity	Survivin (71G4B7E) Rabbit mAb detects endogenous levels of total Survivin protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 60 of human Survivin.
Background	Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).

Specifications