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Cell Signaling Bim (C34c5) Rabbit mAb
List Price
$146.45
Your Price
$146.45
Cell Signaling Bim (C34c5) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-2933T
MFG.PART:
2933T
UNSPSC:
12352202
Manufacturer:
Cell Signaling
| Size | 20 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 12, 15, 23 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Simple Western™: 1:50 - 1:250, Immunoprecipitation: 1:200, IHC Leica Bond: 1:100 - 1:400, Immunohistochemistry (Paraffin): 1:100 - 1:400, Immunofluorescence (Immunocytochemistry): 1:100 - 1:200, Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product #26184. |
| Specificity/Sensitivity | Bim (C34C5) Rabbit mAb detects endogenous levels of total Bim (EL, L and S isoforms) protein. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of Bim. |
| Background | Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1 (1,2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL, and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity. |
| SKU | CSIG-2933T |
|---|---|
| Supplier Part Number | 2933T |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |