Cell Signaling Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific)

Name: Cell Signaling Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific)
SKU: CSIG-9429T
Price: 147.46 USD

List Price $147.46 Your Price $147.46

Cell Signaling Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9429T

MFG.PART: 9429T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	M
Sensitivity	Endogenous
Molecular Weight (kDa)	18, 43
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved Caspase-8 (Asp387) Antibody detects endogenous levels of mouse and rat caspase-8 when cleaved at Asp387. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43) as well as the p18 subunit alone.
Species Reactivity	Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to the Asp387 cleavage site of rodent caspase-8. Antibodies were purified by protein A and peptide affinity chromatography.
Background	Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

Specifications