Cell Signaling Caspase-9 (C9) Mouse mAb

Name: Cell Signaling Caspase-9 (C9) Mouse mAb
SKU: CSIG-9508T
Price: 144.43 USD

List Price $144.43 Your Price $144.43

Cell Signaling Caspase-9 (C9) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9508T

MFG.PART: 9508T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R Hm Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	47/37/35 (H). 49/39/37 (M). 51/40/38 (R).
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Caspase-9 (C9) Antibody detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments.
Species Reactivity	Human, Mouse, Rat, Hamster, Monkey
Source/Purification	Monoclonal Antibody is produced by immunizing mice with a recombinant human caspase-9 protein.
Background	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Specifications