Cell Signaling Cleaved Parp (Asp214) (19f4) Mouse mAb (Human Specific)

Name: Cell Signaling Cleaved Parp (Asp214) (19f4) Mouse mAb (Human Specific)
SKU: CSIG-9546T
Price: 164.63 USD

List Price $164.63 Your Price $164.63

Cell Signaling Cleaved Parp (Asp214) (19f4) Mouse mAb (Human Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9546T

MFG.PART: 9546T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	89
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:2000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) detects endogenous levels of the large fragment (89 kDa) of PARP1 resulting from cleavage at aspartic acid 214. This antibody will detect high levels of full length PARP.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in human PARP.
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

Specifications