Takara pCold I DNA \25 ug

Takara Takara Bios pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors (including pCold I) contain a lac operator (for control of expression), ampicillin resistance gene (amp^r), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). pCold I also contains a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bios Chaperone Plasmid Set (Cat.# 3340).Note: Licensing agreement is required. Please see our website for information. This product is for research use only. It must not be used for clinical purposes or in vitro diagnosis. - TAKBGN (Additional S&H or Hazmat Fees May Apply)