Sigma-Aldrich Readycult Coliforms 100 1x

Agency

EPA (Approved as alternative method)

Quality Level

200

Sterility

sterile; γ-irradiated

Form

medium granules (dehydrated (DCM))

Manufacturer/Tradename

ReadyCult^®

Technique(S)

microbiological culture: suitable

Ph

6.6-7.0 (25 °C, 17 g/L in H₂O)

Solubility

17 g/L

Application(S)

food and beverages
microbiology

Storage Temp.

15-25°C

Related Categories

Microbial Culture Media

General Description

Coliforms 100 is a chromogenic medium that provides easy-to-read, definitive positive results. It has a high nutritional quality of the peptones along with incorporated phosphate buffer that guarantees rapid growth of coliforms, while lauryl sulfate largely inhibits the accompanying flora (especially the Gram-positive). The chromogenic substrates X-GAL (cleaved by coliforms) and the fluorogenic substrate MUG (highly specific forE. coli) allow for the simultaneous detection of total coliforms andE. coli. The presence of total coliforms is indicated by a blue-green color of the broth andE. coliby a blue fluorescence under UV-light. The optional 30-second indole reaction helps to accurately confirm the presence ofE. coli. ReadyCult^®tests are convenient, rapid and economical.

Application

Coliforms 100 is a rapidE. coli/Coliform test for drinking water. It is a selective enrichment broth used for the simultaneous presence / absence detection of total coliforms andE. coliwithin the bacteriological water examination.

Linkage

Replaces: 1012980002

Analysis Note

Appearance
- clearness:
clear
- colour: yellowish
pH-value (25 °C): 6.6 - 7.0
Inoculum on reference medium
- Escherichia coli ATCC 11775 (WDCM 00090):
10 - 100
- Citrobacter freundii ATCC 8090: 10 - 100
- Klebsiella pneumoniae ATCC 31488: 10 - 100
- Salmonella typhimurium ATCC 14028 (WDCM 00031): 10 - 100
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): ≥ 1000
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): +
colour change to blue-green
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Fluorescence
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Indole formation
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
Incubation: 24 hrs.; 35 °C; aerobic.READYCULT is a registered trademark of Merck KGaA, Darmstadt, Germany