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PluriSTEM™ Recombinant Vitronectin is an effective xeno-free alternative to matrigel™ and has been validated to successully culture human ES and iPS cells using PluriSTEM™ Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.
PluriSTEM™ Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.
Product Information
Presentation
Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.
Quality Level
MQ100
Applications
Application
Human pluripotent stem cell culture.
Key Applications
Cell Attachment
Cell Culture
Stem Cell Culture
Application Notes
Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.
Biological Information
Concentration
0.5 mg/mL
Host
E. coli
Purity
Product is ≥ 92% pure by SDS-PAGE.
Gene Symbol
VTN
UniProt Number
P04004
Stem Cell Type
Human Embryonic Stem Cells
Product Usage Statements
Quality Assurance
Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.
