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LI-COR IRDye® 800cw Streptavidin, 0.5 mg
List Price
$166.05
Your Price
$166.05
LI-COR IRDye Streptavidin, 800CW Series, 0.5 mg Net Content, 1 mg/mL, Liquid Form, Green, 4 deg C - LIC (Additional S&H or Hazmat Fees May Apply)
NETA PART:
LIC-926-32230
MFG.PART:
926-32230
UNSPSC:
12352200
Manufacturer:
LI-COR BioScience
IRDye 800CW Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
Recommended Dilutions
| Application | Suggested Range | Tween 20* | SDS* |
|---|---|---|---|
| Odyssey® Western blot detection | 1:1,000 - 1:5,000 | 0.1 - 0.2% (v/v) | 0.02 - 0.1% (v/v) |
| Other | User optimized | User optimized | User optimized |
Optimum dilutions will vary and should be determined empirically.
* Added to reduce non-specific background staining.
See the complete line of IRDye Streptavidins.
| SKU | LIC-926-32230 |
|---|---|
| Supplier Part Number | 926-32230 |
| UM | EA |
| UNSPSC | 12352200 |
| Manufacturer | LI-COR BioScience |
| CountryOfOrigin | United States |
| ProductLine | LIC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |