Quality Bio low-EDTA TE, 1X pH 8.0 4x100ml

Description

Low EDTA TE (1X), pH 8.0 is molecular biology grade TE buffer with 0.1mM EDTA and is used to store DNA and RNA.

EDTA chelates Mg⁺² and other divalent metal ions – (inhibits DNase and RNase to suppress DNA and RNA degradation). Some enzymes, such as Taq polymerase used in PCR, require a certain level of Mg⁺² for enzymatic activity. This version of TE buffer, with 10X less EDTA, does not interfere with the Mg⁺² in the reactions.

Tris is a buffering agent to keep the solution at a defined pH – The ‘Cl’ or ‘HCl’ indicates the pH was attained using aqueous hydrochloric acid (HCl), since Tris is naturally basic. Tris–EDTA (TE) is routinely used for suspending nucleic acid samples.

• With 0.1 mM EDTA
• Filtered through a 0.1 µm membrane
• DNase, RNase, and Protease tested

Applications

Low EDTA is recommended to re-suspend final purified DNA when DNA is used for downstream PCR reactions.

Storage Conditions: 15°C to 30°C

Shipping Condition: ambient temperature

Sterile Filtered

pH: 8.0 ± 0.1

Max Shelf Life: 36 months from date of manufacture

Harmonization Code: 3822.00.5090

ECCN: EAR99