Cell Signaling Phospho-Gsk-3beta (Ser9) (D2y9y) Mouse mAb

Name: Cell Signaling Phospho-Gsk-3beta (Ser9) (D2y9y) Mouse mAb
SKU: CSIG-14630S
Price: 400.97 USD

List Price $400.97 Your Price $400.97

Cell Signaling Phospho-Gsk-3beta (Ser9) (D2y9y) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-14630S

MFG.PART: 14630S

UNSPSC: 41116113

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	46
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-GSK-3β (Ser9) (D2Y9Y) Mouse mAb recognizes endogenous levels of GSK-3β protein only when phosphorylated at Ser9. This antibody does not cross-react with GSK-3α when phosphorylated at Ser21.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β protein.
Background	Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

Specifications