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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT45 |
| Description | Anti-Claudin-5 Antibody |
| Alternate Names |
|
| Background Information | Claudin-5 (UniProt: O00501; also known as Transmembrane protein deleted in VCFS, TMDVCF) is encoded by the CLDN5 (also known as AWAL, TMVCF) gene (Gene ID: 7122) in human. Claudin-5 is a multi-pass membrane protein with three cytoplasmic domains (aa 1-7; 103-122; and 181-218); four transmembrane domains (aa 8-28, 82-102, 123-143, and 160-180); and two extracellular loops (aa 29-81 and 144-159). It plays a major role in tight junction-specific obliteration of the intercellular space. It is localized specifically to the endothelial cell layer in the brain, lung, liver, kidney, and skin. In peripheral tissues, it displays greater heterogeneity in tissue expression. At the blood-brain barrier, it is one of the most enriched tight junction proteins and its dysfunction has been linked to several neurodegenerative diseases, including Alzheimer s disease and multiple sclerosis. Cldn5-null mice are reported to die shortly after birth. (Ref.: Hashimoto, Y., et al. (2018). Sci. Rep. 8; 8383; Greene, C., et al. (2019). Fluids Barriers CNS. 16;3). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Claudin-5, Cat. No. ABT45, is a rabbit polyclonal antibody that detects Claudin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Claudin-5 in Mouse and Rat Lung tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Claudin-5 in human prostate tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Claudin-5. |
| Epitope | Cytoplasmic Domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Claudin-5. It targets an epitope within 12 amino acids from the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Horse, Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Plays a major role in tight junction-specific obliteration of the intercellular space (By similarity). SUBUNIT STRUCTURE: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity). SUBCELLULAR LOCATION: Cell junction › tight junction. Cell membrane; Multi-pass membrane protein. SEQUENCE SIMILARITIES: Belongs to the claudin family. |
| Molecular Weight | ~17 kDa observed; 23.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Human Lung tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Claudin-5 in Human lung tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |