Revvity Alphalisa Surefire Ultra Vegfr2 Py1175 Lysate

Revvity This Control Lysate Is Intended For Use With The Alphalisa Surefire Ultra Phospho-Vegf Receptor 2 (Tyr1175) Assay Kit, Or For Use As A Control For Those Running Orthogonal Or Secondary Assays Along With The Surefire Assay. This Control Lysate Was Prepared From Huvec Cells Grown To Confluence In T175 Flasks, And Then Serum-Starved Overnight. The Following Day The Media Was Removed, And The Cells Were Treated With 100 Ng/Ml Vegf For 10 Minutes. The Agonist Was Removed, And The Cells Were Lysed With 4 Ml Of Freshly Prepared 1x Lysis Buffer, With Shaking For 10 Minutes.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)