We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Cell Signaling Phospho-Rb (Ser608) Antibody
List Price
$147.46
Your Price
$147.46
Cell Signaling Phospho-Rb (Ser608) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-2181T
MFG.PART:
2181T
UNSPSC:
41116111
Manufacturer:
Cell Signaling
| Size | 20 µl |
| Reactivity | H R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 110 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-Rb (Ser608) Antibody detects endogenous levels of Rb only when phosphorylated at serine 608. |
| Species Reactivity | Human, Rat, Monkey |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser608 of human Rb. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). |
| SKU | CSIG-2181T |
|---|---|
| Supplier Part Number | 2181T |
| UM | EA |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |