Cell Signaling P27 Kip1 Antibody

Name: Cell Signaling P27 Kip1 Antibody
SKU: CSIG-2552T
Price: 132.31 USD

List Price $132.31 Your Price $132.31

Cell Signaling P27 Kip1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2552T

MFG.PART: 2552T

UNSPSC: 41116111

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	27
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	p27 Kip1 Antibody detects endogenous levels of total p27 protein. The antibody may recognize p57 Kip2
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse p27. Antibodies are purified by protein A and peptide affinity chromatography.
Background	p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).

Specifications