We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
Cell Signaling Mlh1 (4c9c7) Mouse mAb
Cell Signaling Mlh1 (4c9c7) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$138.00
Your Price
$138.00
HOW MUCH YOU SAVE:
0.00 %
| NETA PART: | CSIG-3515T |
| MFG.PART: | 3515T |
| UNSPSC: | 41116111 |
| Manufacturer: | Cell Signaling |
| Size | 20 µl |
| Reactivity | H Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 85 |
| Source/Isotype | Mouse IgG1 |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:300, Flow Cytometry (Fixed/Permeabilized): 1:400} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein. |
| Species Reactivity | Human, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with truncated recombinant MBP-MLH1. |
| Background | Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7). |
| SKU | CSIG-3515T |
|---|---|
| Supplier Part Number | 3515T |
| UM | UNIT |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |