Cell Signaling Mlh1 (4c9c7) Mouse mAb

Name: Cell Signaling Mlh1 (4c9c7) Mouse mAb
SKU: CSIG-3515T
Price: 144.43 USD

List Price $144.43 Your Price $144.43

Cell Signaling Mlh1 (4c9c7) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3515T

MFG.PART: 3515T

UNSPSC: 41116111

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	85
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:300, Flow Cytometry (Fixed/Permeabilized): 1:400}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with truncated recombinant MBP-MLH1.
Background	Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).

Specifications