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Cell Signaling Mcm7 (D10a11) Xp Rabbit mAb
Cell Signaling Mcm7 (D10a11) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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| NETA PART: | CSIG-3735T |
| MFG.PART: | 3735T |
| UNSPSC: | 41116111 |
| Manufacturer: | Cell Signaling |
| Size | 20 µl |
| Reactivity | H M R Hm Mk Dg |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunohistochemistry (Paraffin): 1:200 - 1:800, Immunofluorescence (Immunocytochemistry): 1:100 - 1:200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #58963. |
| Specificity/Sensitivity | MCM7 (D10A11) XP® Rabbit mAb detects endogenous levels of total MCM7 protein. Western blot analysis and immunofluorescent data indicate that the antibody is more reactive to primate than rodent proteins. |
| Species Reactivity | Human, Mouse, Rat, Hamster, Monkey, Dog |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human MCM7. |
| Background | The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading in vitro (8). Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication (9). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,10). |
| SKU | CSIG-3735T |
|---|---|
| Supplier Part Number | 3735T |
| UM | UNIT |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |