Cell Signaling Cyclin A2 (Bf683) Mouse mAb

Name: Cell Signaling Cyclin A2 (Bf683) Mouse mAb
SKU: CSIG-4656T
Price: 144.43 USD

List Price $144.43 Your Price $144.43

Cell Signaling Cyclin A2 (Bf683) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4656T

MFG.PART: 4656T

UNSPSC: 41116111

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	55
Source/Isotype	Mouse IgE
Application/Dilution	{Western Blotting: 1:2000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cyclin A2 (BF683) Mouse mAb detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin A2 protein.
Background	While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of overexpressed cyclin A to accelerate the G1 to S transition causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).

Specifications