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Cell Signaling Phospho-Cdc25c (Ser216) (63f9) Rabbit mAb
Cell Signaling Phospho-Cdc25c (Ser216) (63f9) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$157.00
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$157.00
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| NETA PART: | CSIG-4901T |
| MFG.PART: | 4901T |
| UNSPSC: | 41116111 |
| Manufacturer: | Cell Signaling |
| Size | 20 µl |
| Reactivity | H Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:100} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-cdc25C (Ser216) (63F9) Rabbit mAb detects endogenous levels of cdc25C only when phosphorylated at Ser216. |
| Species Reactivity | Human, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. |
| Background | Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). |
| SKU | CSIG-4901T |
|---|---|
| Supplier Part Number | 4901T |
| UM | UNIT |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |