We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
Cell Signaling Phospho-Rb (Ser795) Antibody
Cell Signaling Phospho-Rb (Ser795) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$144.00
Your Price
$144.00
HOW MUCH YOU SAVE:
0.00 %
NETA PART: | CSIG-9301T |
MFG.PART: | 9301T |
UNSPSC: | 41116111 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H Mk |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 110 |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | Phospho-Rb (Ser795) Antibody detects endogenous levels of Rb only when phosphorylated at Ser795. The antibody does not cross-react with Rb phosphorylated at other sites. |
Species Reactivity | Human, Monkey |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser795 of human Rb. Antibodies are purified by protein A and peptide affinity chromatography. |
Background | The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). |
SKU | CSIG-9301T |
---|---|
Supplier Part Number | 9301T |
UM | UNIT |
UNSPSC | 41116111 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |