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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IF, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5603 |
| Replaces | AB5770 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Sox2 Antibody |
| Alternate Names |
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| Background Information | Transcription factor SOX-2 (UniProt: P48431; also known as SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2) is encoded by the SOX2 gene (Gene ID: 6657) in human. SOX-2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is shown to be critical for early embryogenesis and for embryonic stem cell pluripotency. It may function as a switch in neuronal development. It allows neural cells in undifferentiated state by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Sumoylation of SOX-2 is shown to inhibit binding on DNA and negatively regulate the FGF4 transactivation. Defects in SOX2 gene are linked to microphthalmia that is characterized by eye malformation ranging from small eye size of a single eye to complete bilateral absence of ocular tissue. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SOX2 Antibody, Cat. No. AB5603, is a highly specific rabbit polyclonal antibody SOX2 and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells. Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151). Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138). Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinsons disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395). Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065). Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120). Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557). Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2. |
| Epitope | C-terminal region. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes Sox2 C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat, Bovine, Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse embryonic stem cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected SOX2 in 10 µg of mouse embryonic stem cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |