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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ELISA, WB | Ch | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5745 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Opsin Antibody, Red/Green |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Opsin using this Anti-Opsin Antibody, Red/Green validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Immunoblotting: 1:500:1:2,000 on mouse brain extract. To minimize background staining it is suggested that you include a higher content of detergent (Tween-20) in your dilution and washing solutions. Immunohistochemistry: not tested. It is recommended that you try the antibody at 1:50-1:1,000. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptides from mouse green opsin and zebra finch red opsin. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Chicken |
| Specificity | Opsin, red and green. The antibody reacts with a protein of ~40 kDa. |
| Species Reactivity |
|
| Species Reactivity Note | Rat and mouse. Other species have not been tested. It is expected that the antibody will also react with zebra finch. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called red cone photopigment or long-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. This gene and the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of partial, protanopic colorblindness. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P04000 # Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. SIZE: 364 amino acids; 40572 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: The three color pigments are found in the cone photoreceptor cells. PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. DISEASE:SwissProt: P04000 # Defects in OPN1LW are the cause of partial colorblindness protan series (CBP) [MIM:303900]; also called protanopia.SIMILARITY:SwissProt: P04000 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |