Cytiva GenomiPhi V3 Ready-To-Go DNA Amplification Kit, Reaction Time: 1.5 to 2 hr,20 deg C, 480 Reactions

Cytiva GenomiPhi V3 Ready-To-Go DNA Amplification Kit, Reaction Time: 1.5 to 2 hr, -20 deg C, 480 Reactions. GenomiPhi V3 Ready-To-Go DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for whole genome amplification by isothermal strand displacement amplification. The reaction mixture is provided as pre-dispensed, single-dose, lyophilized cakes in either strips of 8 tubes or 96 well plates. Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. The average product length is over 10 kb. The GenomiPhi V3 Ready-To-Go DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 12 to 20 ug DNA can be achieved in less than 2 hr from only 10 ng of genomic DNA input. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method or a non-purified cell lysate may be used. Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual. GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing and DNA archival - GHC-F (Additional S&H or Hazmat Fees May Apply)