Takara pTV 118N DNA (0.5 OD) \25 ug

Takara pTV118N DNA contains the sequence CCATGC, which is the NcoI cleavage site and contains the initiation codon (ATG) of lacZ. When a target gene is inserted into the NcoI site, induction and expression of the gene will be under control of the lac promoter and SD sequence of lacZ. This vector is designed to have a distance of 8 bases between the SD sequence of lacZ and the starting codon, in order to improve the efficiency of translational initiation. The translation frame can be confirmed by sequencing the starting codon region using M13 primer RV-N after isolation of the single-strand DNA using helper phage. Note: It is not possible to sequence the single-stranded DNA using a typical M13 forward primer, because the M13 IG region for lacZ in pTV118N is reverse-oriented relative to pUC118. - TAKBGN (Additional S&H or Hazmat Fees May Apply)