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GenScript Benz-Neburase™ Nuclease Elisa Kit; 96Tests
List Price
$498.00
Your Price
$498.00
GenScript Benz-Neburase™ Nuclease Elisa Kit - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-L00886
MFG.PART:
L00886
UNSPSC:
41116126
Manufacturer:
GenScript USA Inc
Note
For research use only. Not for use in diagnostic procedures.
Overview
| Product Description | GenScript Benz-Neburase™ Nuclease ELISA Kit is a sensitive assay to detect and quantify residual endonuclease impurity in purification and manufacturing processes of viral vectors and vaccines where utilized Benz-Neburase™ Nuclease or other Serratia marcescens endonucleases. Benz-Neburase™ Nuclease ELISA Kit is based on sandwich ELISA with a detection limit of 8.40 pg/ml. It is a simple, accurate and reproducible solution for residual endonuclease quantitation to add to the purification process, quality control, and release testing. |
Product Introduction
| Linearity Range | 15.63 pg/ml-1000 pg/ml | |||||||||||||||||||||||||||||||||||||||
| Sensitivity | 8.40 pg/ml | |||||||||||||||||||||||||||||||||||||||
| Kit Contents |
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| Storage | The unopened kit is stable for at least 12 months from the date of manufacture if stored at 2 °C to 8 °C, and the opened kit is stable for up to 1 month from the date of opening at 2 °C to 8 °C. Do not freeze the reagents in this kit. |
Applications
| Detect and quantify residual endonuclease impurity in purification and manufacturing processes of viral vectors and vaccines where utilized Benz-Neburase™ Nuclease or other Serratia marcescens endonucleases. |
Assay Principle
| Assay Principle | When Benz-Neburase™ Standard or testing samples are added to the Capture Plate, Benz-Neburase™ Nuclease in the Standard or testing samples can be captured by the Anti- Benz-Neburase™ Nuclease antibody which is coated on the Capture Plate. Then the Detection Reagent (containing Anti- Benz-Neburase™ Nuclease antibody conjugated with biotin) is added to interact with the Benz-Neburase™ Nuclease bound on the plate. Enzyme Conjugate (Streptavidin-Horseradish Peroxidase conjugate) is added to interact with the biotin conjugated anti- Benz-Neburase™ Nuclease antibody. After the washing steps, TMB Solution (3, 3’, 5, 5’-Tetramethylbenzidine solution) is added resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color (Optical density, OD) can be measured at 450 nm and 650 nm by a microplate reader. The amount of Benz-Neburase™ Nuclease in the sample is positively proportional with the OD value and can be calculated using a standard curve generated using the Benz-Neburase™ Standard. |
For research use only. Not for use in diagnostic procedures.
| SKU | GSCRPT-L00886 |
|---|---|
| Supplier Part Number | L00886 |
| UM | EA |
| UNSPSC | 41116126 |
| Manufacturer | GenScript USA Inc |
| MSDS URL | Click here |
| Temperature | The unopened kit is stable for at least 12 months from the date of manufacture if stored at 2 °C to 8 °C, and the opened kit is stable for up to 1 month from the date of opening at 2 °C to 8 °C. Do not freeze the reagents in this kit. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | Y |
| Energy Star | No |
| Green | No |
| Controlled | N |