We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
GenScript Bsa Elisa Kit, 2g; 96 tests
List Price
$498.00
Your Price
$498.00
GenScript Bsa Elisa Kit, 2g (Cat. No. L00976) Is A Sandwich Elisa Kit That Is Designed For Quantitative Measurement Of Bovine Serum Albumin (Bsa) In Testing Samples - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-L00976
MFG.PART:
L00976
UNSPSC:
41116126
Manufacturer:
GenScript USA Inc
Overview
| Product Description | GenScript BSA ELISA Kit, 2G (Cat. No. L00976) is a Sandwich ELISA Kit that is designed for quantitative measurement of bovine serum albumin (BSA) in testing samples. These outstanding features make it a great solution for routine BSA impurity detection, quality control and lot release, as well as purification process optimization. |
Product Introduction
| Linearity Range | 0.5 ng/mL - 35 ng/mL | ||||||||||||||||||||||||||||||
| Sensitivity | LoB: 0.14 ng/mL LoQ: 0.5 ng/mL | ||||||||||||||||||||||||||||||
| Kit Contents |
| ||||||||||||||||||||||||||||||
| Storage | The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C. |
Assay Principle
| Assay Principle | BSA ELISA Kit, 2G utilizes two anti-BSA monoclonal antibodies which bind to different epitopes of BSA. When the sample and the anti-BSA detection antibody conjugated with biotin are added to the plate (Capture Plate) coated with the anti-BSA capture antibody, the capture antibody and the detection antibody (Biotin Anti-BSA Antibody) bind to the BSA in the sample in a sandwich format. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the Biotin Anti-BSA Antibody. After washing steps, 3,3,5,5-Tetramethylbenzidine solution (TMB Solution) is added, resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm and 650 nm by a microplate reader. The quantity of BSA in the sample is precisely quantified against a BSA standard curve.  Schematic diagram of BSA ELISA Kit, 2G |
| Reference | 1, Francis G L. Albumin and mammalian cell culture: implications for biotechnology applications [J]. Cytotechnology, 2010, 62(1): 1-16. 2, CLSI document EP06-A (Evaluation of the Linearity of Quantitative Measurement Procedures: A Statistical Approach; Approved Guideline, 2nd Edition). 3, CLSI document EP17-A2 (Evaluation of Detection Capability for Clinical Laboratory Measurement Procedures; Approved Guideline—Second Edition). |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
| SKU | GSCRPT-L00976 |
|---|---|
| Supplier Part Number | L00976 |
| UM | EA |
| UNSPSC | 41116126 |
| Manufacturer | GenScript USA Inc |
| Temperature | The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | Y |
| Energy Star | No |
| Green | No |
| Controlled | N |