| Description | |
|---|---|
| Catalogue Number | CC1010 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | GM6001 MMP Inhibitor |
| Overview | Specificity: GM6001 MMP inhibitor, also known as Ilomastat or N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide, is a potent inhibitor of collagenases. Molecular weight: 388.47 Reported Ki values are as follows (see Galardy Ref):· Human MMP-1 (Fibroblast collagenase): 0.4 nM· Human MMP-3 (Stromelysin): 27 nM· Human MMP-2 (72 kDa gelatinase): 0.5 nM· Human MMP-8 (Neutrophil collagenase): 0.1 nM· Human MMP-9 (92 kDa gelatinase): 0.2 nM Typical working dilution for use in cultured cells is 10-25 μM. GM6001 will precipitate from aqueous solutions exceeding 100μM. Aqueous dilutions should be performed using low salt buffers to prevent precipitation of GM6001 from solution (Recommended dilution buffer: 50 mM Tris pH7.5, 150 mM NaCl, and 20 mM CaCl2.2H20). It is recommended that the concentration of GM6001 in aqueous working solutions is determined upon dilution and confirmed prior to each use. Additional Application information: Use in vivo Typical dosage for GM6001 in animals is 50-100mg/kg body weight. It is generally administered IP every day or every other day. It is thought to cross the blood brain barrier since studies have shown it effectiveness in MS models. If delivered by I.V. the half life in an animal is approximate an hour so it will have to be administered quite often. Thus the preferred way is to deliver the drug by I.P as a suspension in water or saline. The half life does not change but because it is dissolving in the animals system slowly, administration is not done as frequently. It has to be redelivered every other day or so. It can also be delivered subcutaneously in a saline suspension. The drug will precipitate as stay as a mass within the tissue, but surprisingly enough does get into the animal system to be effective. Solubility Soluble up to at least 400mg/ml in DMSO. It can then be diluted in water and must be mixed very quickly to avoid precipitation. Stability GM6001 in DMSO or Water at a concentration of 1mM (0.1mM is preferred) decomposes at the rate of 1% per month at 4%. At 37C this increases to 1% per day. At -20 it is quite stable and as a solid very highly stable Low salt buffers are recommended as diluents to reduce precipitation. Diluted solutions should never be frozen. Concentration of the stock solution should be periodically determined by absorbance at 280nm. |
| Product Information | |
|---|---|
| Presentation | Provided at 1 mg/mL (2.5 mM) in DMSO. |
| Quality Level | MQ100 |
| Applications | |
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| Application | The GM6001 MMP Inhibitor controls the biological activity of MMP. This small molecule/inhibitor is primarily used for Biochemicals applications. |
| Key Applications |
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| Biological Information | |
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| Concentration | 1 mg/mL (2.5 mM) in DMSO |
| Entrez Gene Number |
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| Entrez Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. |
| Gene Symbol |
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| Protein Target | Interstitial collagenase |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P22894 # Can degrade fibrillar type I, II, and III collagens. COFACTOR: Binds 3 calcium ions per subunit. & Binds 2 zinc ions per subunit. SIZE: 467 amino acids; 53412 Da SUBCELLULAR LOCATION: Cytoplasmic granule. Note=Stored in intracellular granules. TISSUE SPECIFICITY: Neutrophils. DOMAIN: SwissProt: P22894 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain frozen at -20°C in DMSO for up to 12 months. Avoid storage once diluted into aqueous solutions. |
| Packaging Information | |
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| Material Size | 10 mg |