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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Mk, Ca, Fg | ICC | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-330-AF488 |
| Description | Anti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate |
| Alternate Names |
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| Background Information | Partitioning defective 3 homolog (UniProt Q8TEW0; also known as ASIP, Atypical PKC isotype-specific-interacting protein, Bazooka, CTCL tumor antigen se2-5, Par-3 family cell polarity regulator alpha, Par-3 partitioning defective 3 homolog, PAR3-alpha, Protein phosphatase 1 regulatory subunit 118) is encoded by the PARD3 (also known as Baz, PAR3, PAR3A, PARD3A, PPP1R118) gene (Gene ID 56288) in human. Par3 is a PDZ (PSD95/Dlg/ZO-1) domain-containing membrane scaffold adapter protein that plays an important role in epithelial cell tight junction (TJ) formation and apico-basal polarity maintenance. Together with PAR6, atypical protein kinase C (aPKC), and the Rho GTPase CDC42, Par3 forms a complex that regulates cytoskeleton re-organization, post-golgi vesicle trafficking, microtubule network polarization, and asymmetric cell division. PAR6 interacts directly with aPKC isoforms iota/lambda and zeta to form the complex core. Recruitment of GTP-bound CDC42 to the PAR6-aPKC core induces aPKC phosphorylation/activation by PAR6, and subsequent PAR3 binding. Another quaternary apical junctional complex (AJC), Willin–Par6-aPKC–Par3, has also been reported. In this case, the complex-associated aPKC functions to phosphorylate ROCK, resulting in ROCK dissociation from the AJC and thereby preventing excessive ROCK-dependent apical constriction. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Partitioning-defective 3. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Partitioning-defective 3 in A431, and NIH/3T3 cells. The unconjugated antibody (Cat. No. 07-330) is shown to be suitable also for Western blotting, immunocytochemistry, and immunohistochemistry applications. |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with all 11 spliced isofoms (UniProt Q8TEW0-1 to Q8TEW0-11). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~180, 150, 100 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HUVECs. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Partitioning-defective 3 in HUVECs. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |