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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | ICC | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-449-AF488 |
| Description | Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate |
| Alternate Names |
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| Background Information | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3. |
| Key Applications |
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| Application Notes | The unconjugated antibody (Cat. No. 07-449) is shown to be suitable also for immunohistochemistry, immunocytochemistry, immunoprecipitation, multiplex assay, Western blotting, chromatin immunoprecipitation (ChIP), and ChIP-seq applications. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(Kme3)SAP… in which Kme3 corresponds to trimethyl-lysine at residue 27 of human histone H3. |
| Epitope | H3K27me3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | H3K27me3 specificity of the unconjugated antibody (Cat. No. 07-449) has been confirmed using the Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665). |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Rat. Broad species cross-reactivity expected. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~16 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in A431 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |