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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5674P |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Acid Sensitive Ion Channel 1 Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Acid Sensitive Ion Channel 1 using this Anti-Acid Sensitive Ion Channel 1 Antibody validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. Immunohistochemistry: not tested. It is recommended that the antibody be tried at 1-20 μg/mL on paraformaldehyde fixed tissue. ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide (Catalog number AG394) per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 20 amino acid peptide sequence near the extracellular N-terminus of rat ASIC1 (Chen et al. 1998; Waldmann et al. 1997; Garcia-Anoveros et al. 1997; Waldmann et al. 1996). The immunogen sequence has 100% homology with human sodium channel 2 or BNaC2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat Acid Sensitive Ion Channel 1 (ASIC1). The immunogen shows no significant sequence homology with ASIC-beta or other ASICs. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P78348 # Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear (By similarity). SIZE: 528 amino acids; 59967 Da SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and ACCN1 (By similarity). Interacts with PRKCABP. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity). TISSUE SPECIFICITY: Expressed in most or all neurons. PTM: Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. SIMILARITY: SwissProt: P78348 ## Belongs to the amiloride-sensitive sodium channel family. MISCELLANEOUS: Potentiated by Ca(2+), Mg(2+), Ba(2+) and multivalent cations. Inhibited by anti-inflammatory drugs like salicylic acid (By similarity). Potentiated by FMRFamide-related neuropeptides. PH dependence may be regulated by serine proteases. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |