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Key Specifications Table
| Key Applications |
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| CULT |
| Description | |
|---|---|
| Catalogue Number | CC077 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Human Collagen Type V |
| Overview | PROPERTIES: Molecular composition: [alpha1(V)]2 alpha2(V), native triple helix. Purity and retention of native helical structure was monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. |
| Product Information | |
|---|---|
| Presentation | Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Biological Information | |
|---|---|
| Concentration | 1 mg/ml |
| Purity | 95% by SDS-PAGE Contaminants: <2% collagen type I, <1% collagen type III, <2% collagen type IV and <0.5% non-collagen proteins. PURIFICATION: Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose. |
| Source | Human placenta, negative for HBsAG and HIV antibodies. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P20908 # Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. SIZE: 1838 amino acids; 183560 Da SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & Sulfated on 40% of tyrosines. DISEASE: SwissProt: P20908 # Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS-I is a connective-tissue disorder characterized by loose-jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar cigarette-paper scars. Inheritance is autosomal dominant. & Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type II (EDS-II) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis. Inheritance is autosomal dominant. SIMILARITY: SwissProt: P20908 ## Belongs to the fibrillar collagen family. & Contains 1 laminin G-like domain. & Contains 1 TSP N-terminal (TSPN) domain. |
| Stem Cell Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze. |
| Packaging Information | |
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| Material Size | 100 µg |