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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| rH | ELISA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1668 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Phospholipase A2 Antibody, clone CH-7 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | In 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Phospholipase A2 using this Anti-Phospholipase A2 Antibody, clone CH-7 validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Immunoblot: 1:500-1:1000; antibody recognizes all isoforms, the cPLA. Alpha (85kDa) typically migrates as 100-110kDa on reducing westerns; beta migrates as 114kDa; antibody will also recognize the sPLA at approximately 14-20kDa. ELISA Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant cPLA2 expressed from the human cPLA2 gene. |
| Clone | CH-7 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Antibody reacts with cytosolic form of PLA2. Antibody will also react with beta isoform. Epitope has been localized within the first 216 aa of the N-terminal Ca++ binding domain of cPLA2 |
| Isotype | IgG2b |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM] |
| Gene Symbol |
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| Modifications |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P04054 # PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. COFACTOR: Binds 1 calcium ion per subunit (By similarity). SIZE: 148 amino acids; 16360 Da SUBCELLULAR LOCATION: Secreted.SIMILARITY:SwissProt: P04054 ## Belongs to the phospholipase A2 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |