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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IH(P) | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1681 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Vimentin Antibody, clone LN-6 |
| Background Information | Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
|
| Presentation | Liquid tissue culture supernatant containing 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Vimentin Antibody, clone LN-6 detects level of Vimentin & has been published & validated for use in IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry on B5 fixed, paraffin embedded tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Nuclei from adult human thymic biopsy specimens. |
| Clone | LN-6 |
| Host | Mouse |
| Specificity | Reacts with human mesenchymal cells and derived sarcomas. Reacts with an epitope of the cytoskeletal protein vimentin but unlike other vimentin antibodies, does not react with human lymphoid cells or derived malignancies. Reactive with endothelium, muscle, fibroblasts, melanocytes, peripheral nerve, sertoli cells, kidney mesangial cells and tubules, osteoblasts and periosteum, non-hematopoietic tumors of mesenchymal origin (all sarcomas), meningiomas and melanomas. |
| Isotype | IgM |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. SIZE: 466 amino acids; 53652 Da SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein. TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitts lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family. |
| Molecular Weight | 57-60 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 1 mL |