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Millipore Anti-Sr-25 Antibody, Clone 1f6.1 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Srp25 & Has Been Tested In Western Blotting & Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
ADP-ribosylation factor-like protein 6-interacting protein 4
ARL-6-interacting protein 4
Aip-4
HSP-975
HSVI-binding protein
SR-15
SRp25
SR-25
Background Information
Also known as ADP-ribosylation factor-like factor 6-interacting protein and ARL6IP4, the SR-25 protein is expressed in certain human organs and is relatively abundant in human testis and thymus. It is observed to mediate the Rac1 signaling pathway and acts as a splicing inhibitor of the Herpes simplex virus (HSVI); however, further research is needed to elucidate its mode of action.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-SR-25 Antibody, clone 1F6.1 is a highly specific mouse monoclonal antibody, that targets SRp25 & has been tested in western blotting & ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SR-25 in HeLa cells treated with Mimosine and Nocodazole.
Dylight® is a registered trademark of Thermo Fisher Scientific.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the C-terminus of human SR-25.
Clone
1F6.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001002251
Gene Symbol
ARL6IP4
Purification Method
Protein G Purified
UniProt Number
Q66PJ3
Molecular Weight
~32 kDa observed. Uniprot describes six isoforms between 22 kDa and 38 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cells treated with Mimosine and Nocodazole.
Western Blotting Analysis: 1 µg/mL of this antibody detected SR-25 in HeLa cells treated with Mimosine and Nocodazole.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.