Takara SPP System II \1 Set

Takara The SPP (Single Protein Production) System utilizes a modified version of Takaras cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5-ACA-3 sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.Note: Licensing agreement is required. Please see our website for information. This product is for research purposes only. It must not be used for clinical purpose or in vitro diagnosis. - TAKBGN (Additional S&H or Hazmat Fees May Apply)