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Agilent Advancebio O-Glycanase
List Price
$335.00
Your Price
$335.00
Agilent AdvanceBio O-Glycanase. Enzyme releases unsubstituted Ser/Thr-linked GalGalNAc from proteins. Can be used with other enzymes to remove complex O-linked structures. Includes 5X reaction buffer A [250 mM sodium phosphate pH 5.0]. - BC
GROUP SKU:
GK80090
| Application | Detection of O-glycosylation in proteins |
| Concentration | ≥1.25 U/mL |
| Enzyme Applications | Analysis of biosynthesis of glycoproteins Bioactivity in O-glycans Detection of O-glycosylation in proteins Epitope and binding site analysis Glycoprotein deglycosylation Structural analysis of O-glycan |
| Enzyme Formulation | 20 mM Tris HCl pH 7.5 containing 25 mM NaCl. |
| Enzyme Source | Recombinant gene from Streptococcus pneumoniae, expressed in E.coli. |
| Enzyme Specific Activity | ≥12 units/mg |
| Enzyme Specificity | The enzyme is highly specific, liberating Galß1-3GalNAc from serine or threonine residues alone or as part of glycopeptides or glycoproteins. There is no apparent preference for serine over threonine. Substitution of the disaccharide core with sialic acid, fucose, N-acetylglucosamine or N-acetylgalactosamine residues, prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. Absence of the galactose residue or the acetamido group on the innermost N-acetylgalactosamine residue also prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. O-Glycanase is included in the Agilent Enzymatic Deglycosylation kit for N-linked and simple O-linked glycans (GK80110), which may be used in conjunction with our the Agilent Extender kit for complex O-linked glycans (GK80115). |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of Galß1-3GalNAca1-p-nitrophenol per minute at 37°C, pH 5.5. |
| Molecular Weight | ~180 kD |
| Unit | 50 mU |
| Volume | 40 µL |
| pH Optimum | 5.5 |
| pH Range | 5-7 |
| SKU | GK80090 |
|---|---|
| Supplier Part Number | GK80090 |
| UM | CS |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| MSDS URL | Click here |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |