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Agilent Gke-5006b-1 Advancebio N-Glycanase (Pngase F), 80 Ul
List Price
$2,076.00
Your Price
$2,076.00
Agilent AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL. Releases intact N-glycans. Supplied as 5 x 200 mU vials. Includes reaction buffers. For a more concentrated formulation, see GKE-5010B or GKE-5020B. - BC
GROUP SKU:
GKE-5006B-1
| Concentration | ≥2.5 U/mL |
| Enzyme Applications | Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure-function studies of N-glycosylated glycoproteins |
| Enzyme Formulation | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5) |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | ≥10 units/mg |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
| Molecular Weight | ~35,000 daltons |
| Unit | 1000 mU |
| Volume | 80 µL |
| pH Optimum | 8.6 |
| pH Range | 7.5-9.5 |
| SKU | GKE-5006B-1 |
|---|---|
| Supplier Part Number | GKE-5006B-1 |
| UM | CS |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |