We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Agilent Gke-5006d Advancebio N-Glycanase (Pngase F), 400 Ul
List Price
$2,076.00
Your Price
$2,076.00
Agilent AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL. Recombinant PNGase F cleaves intact N-glycans from proteins. Supplied at ≥2.5 U/mL. Contact us for reaction buffers. For ≥10 U/mL, see GKE-5010B or GKE-5020B. - BC
GROUP SKU:
GKE-5006D
| Concentration | 2.5 U/mL |
| Enzyme Applications | Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure-function studies of N-glycosylated glycoproteins |
| Enzyme Formulation | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5) |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | ≥10 units/mg |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
| Molecular Weight | ~35,000 daltons |
| Unit | 1 U |
| Volume | 400 µL |
| pH Optimum | 8.6 |
| pH Range | 7.5-9.5 |
| SKU | GKE-5006D |
|---|---|
| Supplier Part Number | GKE-5006D |
| UM | CS |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |