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Agilent Advancebio N-Glycanase-Plus (Pngase F), >10 U/mL
List Price
$1,420.00
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$1,420.00
Agilent AdvanceBio N-Glycanase-plus (PNGase F), ≥10 U/mL. Enzyme releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes reaction buffers, denaturation, and detergent solutions. - BC
GROUP SKU:
GKE-5010B
| Concentration | 10 U/mL |
| Enzyme Applications | To obtain efficient deglycosylation of glycoprotein substrates under nondenaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase-plus and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored. |
| Enzyme Formulation | 20 mM Tris HCl pH 7.5, containing 1 mM EDTA and 50 mM NaCl |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | ≥10 units/mg |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, is useful for deglycosylation under native conditions. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at pH 7.5 and 37°C. |
| Unit | 400 mU |
| Volume | 40 µL |
| pH Optimum | 8.6 |
| pH Range | 7.5-9.5 |
| SKU | GKE-5010B |
|---|---|
| Supplier Part Number | GKE-5010B |
| UM | CS |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| MSDS URL | Click here |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |