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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC60 |
| Replaces | AB1880 |
| Description | Anti-Endostatin Antibody |
| Alternate Names |
|
| Background Information | Endostatin is a proteolytic cleavage product from the C-terminus of Collagen XVIII, which is itself a proteoglycan/collagen found in blood vessel walls and in basement membranes. Endostatin is a compact globular fold-containing protein with a Zn2+ binding site near its amino terminus. Originally identified in conditioned media of murine hemangioendothelioma (EOMA) cells, endostatin is generated from the parent collagen XVIII molecule involving intermediate glycosylated forms and the proteolytic activity of proteases such as cathepsin L, metalloproteases, and elastase. Endostatin has a wide-spread tissue expression and has been shown to co-localize with fibulins, nidogen-2 in basement membranes, and elastic fibers of veins. Endostatin has also been shown to cause a G1 arrest in endothelial cells and to induce apoptosis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Endostatin in mouse lung tissue. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to the nonhelical region 11 of mouse Endostatin. |
| Epitope | Nonhelical Region 11 Domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes Endostatin at the nonhelical region 11 domain. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix (By similarity). TISSUE SPECIFICITY: Expressed in liver, kidney, lung, skeletal muscle and testis. PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. SEQUENCE SIMILARITIES: Belongs to the multiplexin collagen family. Contains 1 FZ (frizzled) domain. Contains 1 TSP N-terminal (TSPN) domain. |
| Molecular Weight | ~28 kDa observed. A ~28 kDa endostatin fragment has been observed in western blot as a result of MMP-7 cleavage of collagen XVIII (Chang, J., et al. (2003). Invest Ophthalmol Vis Sci. 44:832). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Endostatin in 10 µg of A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |