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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE280 |
| Description | Anti-Ars2 Antibody |
| Alternate Names |
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| Background Information | As a component of the nuclear RNA cap-binding complex (CBC), Ars2 is known to play a critical role in miRNA biogenesis by stabilizing and linking capped primary miRNA transcripts with the primary miRNA processing complex containing DROSHA and DGGR8. Unlike other CBC components, Ars2 expression also plays an important role in cell proliferation. In particular, depletion of Ars2 is shown to reduce the levels of several miRNAs, such as miR-21, let-7, and miR-155, which are implicated in cellular transformation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Ars2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect Ars2 also known as Serrate RNA effector molecule homolog, Arsenite-resistance protein 2. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Ars2 in IP. (Alex Achille, Department of Molecular Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida, USA). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human Ars2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. SUBUNIT STRUCTURE: Interacts with NCBP1 and DROSHA. Interacts with CASP8AP2 and ERBB4. TISSUE SPECIFICITY: Ubiquitously expressed. |
| Molecular Weight | ~120 kDa observed. Uncharacterized bands at ~78 kDa and 48 kDa may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Ars2 in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |