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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H, B, Ch, Fg | ICC, IHC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1567A4 |
| Description | Anti-MAG Antibody, clone 513, Alexa Fluor® 488 Conjugate |
| Alternate Names |
|
| Background Information | Myelin-associated glycoprotein (MAG), a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100 kDa, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MAG Antibody, clone 513, Alexa Fluor 488 Conjugate is validated for use in IC, IH for the detection of MAG. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MAG in rat E18 primary cortex cells. |
| Biological Information | |
|---|---|
| Immunogen | L2 epitope carrying glycoprotein extract of 1-2 day old chicken brain |
| Clone | 513 |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Rat and Mouse. Predicted to react with Human, Bovine, Chicken, and Frog based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha2,3- linked sialic acid (By similarity). SIZE: 626 amino acids; 69069 Da SUBUNIT: Binds to RTN4R (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. & Contains 4 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| Molecular Weight | 69 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in adult mouse brain tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MAG in adult mouse brain tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |