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GenScript Tim-3 Fc Chimera, Human; 1mg
List Price
$1,500.00
Your Price
$1,500.00
GenScript Tim-3 Fc Chimera, Human - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-Z03392-1
MFG.PART:
Z03392-1
UNSPSC:
41116127
Manufacturer:
GenScript USA Inc
Product Introduction
| Species | Human | ||||
| Protein Construction |
| ||||
| Purity | > 95% as analyzed by SDS-PAGE | ||||
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | ||||
| Biological Activity | Assay #1: Immobilized TIM-3, hFc, Human at 0.5 μg/ml, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5.0 ng/ml. Assay #2: Immobilized Galectin-9, His, Human at 0.5 μg/ml (100 µl/well) can bind TIM-3, hFc, Human with a linear range of 0.78-6.25 μg/ml. | ||||
| Expression System | HEK 293 | ||||
| Apparent Molecular Weight | 60~65 kDa, on SDS-PAGE under reducing conditions. | ||||
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. | ||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. | ||||
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Background
| Target Background | T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that co-express PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. |
| Synonyms | Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03392-1 |
|---|---|
| Supplier Part Number | Z03392-1 |
| UM | EA |
| UNSPSC | 41116127 |
| Manufacturer | GenScript USA Inc |
| Temperature | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (examp |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |