Sigma-Aldrich Purostar Nh2 5mym Cart250

Product Name

Purospher^®STAR NH2 (5 µm), L × I.D. 250 mm x 4.6 mm LiChroCART^®HPLC cartridge, manuCART^™holder required for use

Material

stainless steel column

Quality Level

100

Agency

suitable for USP L8

Product Line

Purospher^®

Feature

endcapped: no

Packaging

1 ea of

Extent Of Labeling

3.5% Carbon loading

Parameter

250 bar pressure
60 °C max. temp.

Technique(S)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

Related Categories

HPLC Columns

General Description

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher^®STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher^®STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher^®STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher^®STAR NH2, 5 µm LiChroCART^®250-4.6 is a cartridge column which requires a manuCART^™cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher^®STAR NH2 (5 µm) LiChroCART^®4-4 part number 1.50267.0001. LiChroCART^®cartridge columns mounted with a manuCART^™cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher^®STAR NH2, 5 µm Hibar^®RT 250-4.6 HPLC columns are available part number 1.51913.0001.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Features And Benefits

• Ultra-pure silica (99.999%)
• Low silanol activity
• Reproducible results from run-to-run and batch-to-batch

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0Hibar is a registered trademark of Merck KGaA, Darmstadt, GermanyLICHROCART is a registered trademark of Merck KGaA, Darmstadt, GermanyMANUCART is a trademark of Merck KGaA, Darmstadt, GermanyPurospher is a registered trademark of Merck KGaA, Darmstadt, Germany