Sigma-Aldrich Lisorb Rp8 5mym Hi-Rt125-4

Product Name

LiChrosorb^®RP-8 (5 µm) Hibar^®RT 125-4, suitable for HPLC

Material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

Product Line

LiChrosorb^®

Feature

endcapped: no

Parameter

400 bar pressure
60 °C max. temp.

Technique(S)

HPLC: suitable
mass spectrometry (MS): suitable (Low)

Column L × I.D.

12.5 cm × 4 mm

Matrix

fully porous particle

Related Categories

HPLC Columns

General Description

HPLC columnLiChrosorb^®RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar 125-4 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.

Application

• Determination of amphotericin B, liposomal amphotericin B, and amphotericin B colloidal dispersion in plasma by high-performance liquid chromatography.: This study by Egger et al. focuses on the HPLC determination of various forms of amphotericin B in plasma, utilizing LiChrosorb^®RP-8 as the stationary phase. This method ensures accurate monitoring of these antifungal agents in clinical settings (Egger et al., 2001).


• Determination of peptide hydrophobicity parameters by reversed-phase high-performance liquid chromatography.: This research by Rothemund et al. used LiChrosorb^®RP-8 to determine the hydrophobicity of various peptides, providing valuable insights for peptide characterization in biochemical research (Rothemund et al., 1994).


• HPLC determination of doxorubicin, doxorubicinol and four aglycone metabolites in plasma of AIDS patients.: Beijnen et al. employed LiChrosorb^®RP-8 in an HPLC method to quantify doxorubicin and its metabolites in plasma, offering a robust tool for therapeutic drug monitoring in AIDS patients (Beijnen et al., 1991).

Analysis Note

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Toluene): ≥ 55000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.5 - 1.5Hibar is a registered trademark of Merck KGaA, Darmstadt, GermanyLiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany